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ytnA [2020-08-11 09:45:21]
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ytnA [2020-08-11 09:45:21]

alanine permease
locus
BSU_30530
pI
9.91
mw
50.16 kDa
protein length
463 aa Sequence Blast
gene length
1392 bp Sequence Blast
function
uptake of D- and L-alanine
product
alanine permease
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.4|APC superfamily]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.15|Biosynthesis/acquisition of L- and D-alanine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,124,250 3,125,641

    Phenotypes of a mutant

  • a [gene|219586A3F378DC38EA076FD39B99D41136BDE722|ytnA] [gene|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr] double mutant is not viable due to the inability to acquire D-alanine [https://www.biorxiv.org/content/10.1101/562850v2 reference]

    • The protein

    Catalyzed reaction/ biological activity

  • uptake of D- and L-alanine [https://www.biorxiv.org/content/10.1101/562850v2 reference]

    • Protein family

  • [SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

    • Paralogous protein(s)

  • [protein|423AEC9D260BE53AD22851D999244B89BEB3E84D|HutM], [protein|8C4A8961B2514A8AD531AFB104B10C0797D02AC2|YvbW], [protein|9AD0E43119BDC65D5F67CE55CD9417E1E6BAE058|YbxG], [protein|9BFB16C12B31ABD70BBB921BCB9E4201E3BDC92D|RocC], [protein|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|RocE], [protein|B6A920650919D5BBBD75CCA58C30A0D8ECE64DA3|YbgF], [protein|C7FEA1D9C7C96C39CEE83CE3F13D412DE6AFE10E|YdgF], [protein|CE4A49F5CCC2D36C5CE5AD7AE7F0A70F2D4F425C|AapA], [protein|1594BA83CED2DA9F86C1CE2A0DC6E1C9B665EACB|GabP]

    • Structure

  • [PDB|6F34] (from Geobacillus kaustophilus, 21% identity) [pubmed|29416041]

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617|asnB]-[gene|219586A3F378DC38EA076FD39B99D41136BDE722|ytnA]
    description
    [Pubmed|10498721]

    regulation

  • constitutive
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A126 (ytnA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/126 NBRP B. subtilis, Japan]
  • GP1885 (Δ[gene|219586A3F378DC38EA076FD39B99D41136BDE722|ytnA]::''spc'') available in [SW|Jörg Stülke]'s lab
  • BKE30530 ([gene|219586A3F378DC38EA076FD39B99D41136BDE722|ytnA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTCTTCTCCCCTAGA, downstream forward: _UP4_TGACAAAAAGAGCTCCCAGT
  • BKK30530 ([gene|219586A3F378DC38EA076FD39B99D41136BDE722|ytnA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTTCTTCTCCCCTAGA, downstream forward: _UP4_TGACAAAAAGAGCTCCCAGT

    • Expression vector

  • pGP2280: expression of ''ytnA'' by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP2276 (in [SW|pAC5]) (GP2963), available in [SW|Jörg Stülke]'s lab

    • References

  • 10498721,29416041